One-step vs. two-step RT-qPCR—tips for choosing the right protocol
Real-time PCR for mRNA quantitation | BioTechniques
Simple, Inexpensive RNA Isolation and One‐Step RT‐qPCR Methods for SARS‐CoV‐2 Detection and General Use - Graham - 2021 - Current Protocols - Wiley Online Library
Two-step Quantitative RT-PCR Protocol
Highly sensitive and multiplexed one-step RT-qPCR for profiling genes involved in the circadian rhythm using microparticles | Scientific Reports
Real Time PCR Principle, Protocol, Application, Advantages.